Comparative study of anti-inflammatory properties of Hibiscus Sabdarifa Calyx and Malus Domestica with Ibuprofen

Abdullahi Moyosore, Imrana Salisu, Gideon Adamu Shallangwa


Hibiscus Sabdarifa and Malus Domestica are well known and widely used herbs, which contains several interesting bioactive constituents and possesses health promoting properties. This study is made in order to determine the total phenolic and flavonoid contents, evaluate and compare the anti-inflammatory effects of ethanol extracts of the two extracts and a 1:1 blend of the extracts against the denaturation of proteins in vitro. The respective extracts were analyzed for their contents of polyphenols and flavonoids. The test extracts and reference drug (ibuprofen) of varying concentrations were also incubated with egg albumin under controlled experimental conditions and subjected to determination of absorbance to assess the anti-inflammatory property. The results obtained exhibited concentration-dependent inhibition of protein denaturation by both extracts, the blend as well as the reference drug. The EC50 for extracts as well as those for the blend and the reference drug (ibuprofen) were determined by the dose- response curve using GraphPad Prism 5.0 software. 

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