NEUROBEHAVIOURAL ACTIVITY IN MICE IN THE OPEN FIELD MAZE TASK FOLLOWING LONG TERM INGESTION OF COWPEA DIET

Aduema Nil Wadioni, Vidona W.B., Amah A.K.

Abstract


It was therefore the aim of this study to find out whether long term consumption of cooked and uncooked beans (cowpea) diet has effects on some neurobehavioral parameters such as anxiety and fear using Swiss white mice as experimental animals, since cowpea contain serotonin and its precursor 5-Hydroxytryptophan which have neurobehavioral effects. Forty (40) Swiss mice were randomly assigned into four groups. Control (group A)  were fed normal rodent chow, cooked beans (group B) received cooked beans diet (50% w/w), uncooked beans (group C) also received uncooked beans diet (50% w/w), while another set of mice was placed on serotonin precursor (5-HTP)(group D) diet (0.2mg/50g w/w) for thirty days. All the mice had access to clean drinking water. Anxiety and fear were investigated alongside food and water intake and body weight change. Involvement of serotonin pathway was also investigated using the set of mice administered serotonin precursor for comparison with the beans diet fed mice. The open field test was used to assess anxiety &fear related behaviors. The results showed that the Centre square entry and Centre square duration for the cooked and uncooked cowpea diet was statistically higher (P<0.05 and P<0.01respectively) compared to control while the grooming frequency for the cooked, uncooked and 5-HTP was significantly higher (P<0.05., P<0.001) compared to control. Duration of grooming was also significantly higher (P<0.01) compare to control. The frequency of stretch attend posture and defecation was lower in the test group (p<0.05 respectively). Signifying a decrease in the level of anxiety & fear. The administration of serotonin precursor diet (5-HTP) produced similar results as cooked or uncooked beans, thus suggesting that serotonin may be involved in the action of beans on neurobehavioral parameters. In conclusion, long term consumption of cowpea diet causes calmness and sedation. It is therefore likely that it causes sleep, reduction of aggression and muscle tone. If the result of this finding is extrapolated to man, then, cowpea diet can be used to ameliorate post-traumatic stress disorders. 


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MAYFEB Journal of Biology and Medicine
MAYFEB TECHNOLOGY DEVELOPMENT
Toronto, Ontario, Canada
ISSN 2560-6948