Growth Characteristics and Virulence of Non-tropical Isolate of Metarhizium anisopliae against Banana Weevil, Cosmopolites sordidus (Coleoptera: Curculionidae) invitro.

BORISADE ABIODUN OMOTOSO

Abstract


Abstract:                 Growth rate and virulence of a strain of entomopathogenic Metarhizium anisopliae (Ma 275.86DC) against banana weevil, Cosmopolites sordidus was evaluated at ambient temperature (Mean daily temperature=30°C) invitro in Nigeria. The fungus was tested for virulence against the banana weevil, Cosmopolites sordidus at three conidia concentrations (1.0 x105, 1.0×106 and 3.9×106  conidia ml-1)using mortality bioassay system and virulence was measured by the Median Lethal time (LT50). The mean growth rate of the entomopathogenic fungus was 1.7 mm day-1 at ambient temperature. The fungus infected the insects and the onset of mortality was at five days post inoculation. Mortality was concentration dependent and the LT50 reduced from 11.6 to 9.6 days as the conidia concentration was increased from 1.0 x105 to 1.0×106 conidia ml-1. However, further increase in the concentration of conidia to 3.9×106 conidia ml-1 did not increase virulence significantly (p=0.05). The calculated LC50 of the infective conidia was 6.5 x 102 conidia ml-1. Development of secondary conidia was observed on the insect cadaver after 14 days and mycelia was visible at the inter-segments between the head, the thorax and the abdomen.


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MAYFEB Journal of Agricultural Science
Toronto, Ontario, Canada
MAYFEB TECHNOLOGY DEVELOPMENT
ISSN 2371-512X